A more dramatic decline in UV transparency occurs at shorter wavelengths.  Light sources such as high intensity xenon lamps have been used in some photoreactors to irradiate analytes flowing inside these fluoropolymer tubes with shorter wavelengths in order to increase photolysis.  However, the fluoropolymer tubes absorb most of this radiation, become brittle and leak relatively quickly, especially at the higher temperatures produced by these lamps.

2.  Connect the PEEK screw-in Fitting (i.e. male nut inlet) to the chromatographic column outlet or to the outlet of the detector if one is being used upstream from the       PhotoBlaster®  System.  Notice that the fitting was only slipped on to the fluoropolymer tube at the factory;  therefore you must seat it into the bottom of the female fitting and finger tighten the male nut firmly into the tubing to obtain a zero dead volume fitting. Next, turn on the pumping system and allow 1 to 2 ml. of mobile phase to flush through the LuxTube®  Assembly. Then connect the PEEK  Flangeless Nut and ferrule (i.e. male nut outlet ) to the 0.5 micron in-line frit .

3.  Attach the in-line filter to the 10-32 female inlet of the downstream detector.  The stem will self-adjust to the tube stop depth.

4.  Attach the flangeless nut and ferrule outlet of the LuxTube® Assembly to the  in-line filter Fitting inlet by carefully hand tightening it until it bottoms out to obtain a zero dead volume connection.

5.  With the PhotoBlaster® System -1 turned OFF, tilt the instrument and slide the reflector tube from the bottom all the way up into the reflector tube holder until it stops. Do not  put your fingers into the reflector tube holder because the sharp upper end can cut you! Then, set the instrument upright and turn it on. Allow 10 to 15 minutes for your system to  warm up while mobile phase flows through it.

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